Primer

Part:BBa_K5427055

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW _pTac_RBS1234_SS (I GEM_2024_3)

Forward primer for Gibson Assembly of pTac, RBS1/2/3/4, and masP1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 28
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 28
    Illegal NotI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 28
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 28
    Illegal XbaI site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None